Hotspot

rbcL-accD

LSC · 56402-57100 · 699 bp

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Pi 0.0138

MarkerSeek score 63.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 82
pi: 0.0138
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGCTGCTTGTGAAGTATGGA	CAACATCGAATTGAACCACCA	50.0	42.9	58.3	57.1	803-841 bp	0.974359	71.4
rbcL-accD_p2	CGCTGCTTGTGAAGTATGGA	ACAACATCGAATTGAACCACC	50.0	42.9	58.3	57.1	804-842 bp	0.974359	71.4
rbcL-accD_p3	CGCTGCTTGTGAAGTATGGA	GACAACATCGAATTGAACCACC	50.0	45.5	58.3	58.2	805-843 bp	0.974359	69.3
rbcL-accD_p4	AAATGGAGCCCTGAACTAGC	CAACATCGAATTGAACCACCA	50.0	42.9	57.6	57.1	823-861 bp	0.923077	65.0
rbcL-accD_p5	AAATGGAGCCCTGAACTAGC	ACAACATCGAATTGAACCACC	50.0	42.9	57.6	57.1	824-862 bp	0.923077	65.0