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psbM-trnD-GUC

LSC · 30148-31238 · 1091 bp

Back to Muscari

Pi 0.00806

MarkerSeek score 65.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 86
pi: 0.00806
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	468-1235 bp	1.0	68.5
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	ATGCTATCACCTCACTGGGA	45.0	50.0	55.8	57.8	921-1261 bp	0.589744	51.0
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TGCTATCACCTCACTGGGAT	45.0	50.0	55.8	57.8	920-1260 bp	0.589744	51.0
psbM-trnD-GUC_p4	AGTGCGAGAATATTGACTTCCA	ATGCTATCACCTCACTGGGA	40.9	50.0	57.5	57.8	897-1237 bp	0.589744	49.1
psbM-trnD-GUC_p5	AGTGCGAGAATATTGACTTCCA	TGCTATCACCTCACTGGGAT	40.9	50.0	57.5	57.8	896-1236 bp	0.589744	49.1