Hotspot

trnC-GCA-petN

LSC · 28013-28981 · 969 bp

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Pi 0.0114

MarkerSeek score 72.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 99
pi: 0.0114
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	808-1056 bp	1.0	87.7
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	860-1108 bp	1.0	86.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	859-1107 bp	1.0	86.5
trnC-GCA-petN_p4	TTTTGGATCGAATTTGGCGG	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.4	58.0	847-1095 bp	1.0	86.4
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	TTCCCAGCTCTAGAGTCCAC	50.0	55.0	57.5	58.1	873-1121 bp	1.0	86.2