Hotspot

rpoB-trnC-GCA

LSC · 26733-27941 · 1209 bp

Back to Muscari

Pi 0.013

MarkerSeek score 76.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1209
variable_sites: 116
pi: 0.013
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAATTCTTCTGCCAAGCCCT	TTTGTTGATCAGGCGACACC	45.0	50.0	57.7	58.8	1258-1414 bp	0.974359	81.0
rpoB-trnC-GCA_p2	AAATTCTTCTGCCAAGCCCT	GACACCCGGATTTGAACTGG	45.0	55.0	57.7	58.8	1244-1400 bp	0.974359	80.5
rpoB-trnC-GCA_p3	AAATTCTTCTGCCAAGCCCT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.7	57.5	1221-1377 bp	0.923077	80.5
rpoB-trnC-GCA_p4	CTTCTGCCAAGCCCTGATTA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1216-1372 bp	0.923077	79.7
rpoB-trnC-GCA_p5	AATTCTTCTGCCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1220-1376 bp	0.923077	79.5