Hotspot

trnS-GCU-trnG-UCC

LSC · 8340-9337 · 998 bp

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Pi 0.0138

MarkerSeek score 72.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 104
pi: 0.0138
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCGAACCCTCGGTACAAGTA	AACCGAAAGACCCCTTAACT	50.0	45.0	57.8	56.4	997-1143 bp	1.0	77.2
trnS-GCU-trnG-UCC_p2	GGAACGGAGAGAGAGGGATT	AACCGAAAGACCCCTTAACT	55.0	45.0	58.2	56.4	1016-1162 bp	1.0	76.9
trnS-GCU-trnG-UCC_p3	AATCTGCCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	958-1104 bp	1.0	76.8
trnS-GCU-trnG-UCC_p4	CAATCTGCCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	959-1105 bp	1.0	76.8
trnS-GCU-trnG-UCC_p5	CGGAGAGAGAGGGATTCGAA	AACCGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	1012-1158 bp	1.0	76.1