Hotspot

trnK-UUU-rps16

LSC · 4069-4705 · 637 bp

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Pi 0.0135
MarkerSeek score 79.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
637
variable_sites
71
pi
0.0135
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAA GGTGCTCAACCTACAGGAAC 45.0 55.0 58.1 58.2 631-776 bp 1.0 89.5
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.3 58.2 632-777 bp 1.0 84.6
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAA GTGCTCAACCTACAGGAACT 45.0 50.0 58.1 57.2 630-775 bp 1.0 84.3
trnK-UUU-rps16_p4 GTCGCACTTAAAAGCCGAAT GGTGCTCAACCTACAGGAAC 45.0 55.0 57.1 58.2 630-775 bp 1.0 83.3
trnK-UUU-rps16_p5 ACCATTGAGTTAGCAACCCA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.0 58.2 605-750 bp 1.0 82.7