Hotspot

psbA

LSC · 208-1269 · 1062 bp

Back to Musa

Pi 0.00234
MarkerSeek score 28.60
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
10
pi
0.00234
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AACAAAGCAGCAATACCCCA CATAGGGAAAGTCGTGTGCA 45.0 50.0 58.0 57.9 1546-1577 bp 0.458333 61.5
psbA_p2 AACAAAGCAGCAATACCCCA ACATAGGGAAAGTCGTGTGC 45.0 50.0 58.0 57.9 1547-1578 bp 0.458333 61.5
psbA_p3 ACAAAGCAGCAATACCCCAT CATAGGGAAAGTCGTGTGCA 45.0 50.0 57.8 57.9 1545-1576 bp 0.458333 59.7
psbA_p4 AACAAAGCAGCAATACCCCA AATACGTGTGCTTGGGAGTC 45.0 50.0 58.0 57.9 1202-1203 bp 0.458333 58.2
psbA_p5 ACAAAGCAGCAATACCCCAT AATACGTGTGCTTGGGAGTC 45.0 50.0 57.8 57.9 1201-1202 bp 0.458333 56.4