Hotspot

accD

LSC · 60901-62706 · 1806 bp

Pi 0.0175

MarkerSeek score 58.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1806
variable_sites: 118
pi: 0.0175
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GATCCTTGGGATTGGTGGAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	2485-2899 bp	0.979167	89.5
accD_p2	GAAGTGATACCTCGGCTTCC	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.8	58.0	2211-2743 bp	1.0	89.1
accD_p3	GATCCTTGGGATTGGTGGAC	ATTCGGTCCCATTGGGTCTA	55.0	50.0	57.9	58.1	2543-2957 bp	0.979167	88.6
accD_p4	AAGGCTCCTTTACCCATCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2421-2946 bp	0.9375	87.9
accD_p5	AAGGCTCCTTTACCCATCCT	ATTCGGTCCCATTGGGTCTA	50.0	50.0	58.0	58.1	2479-2893 bp	0.916667	86.3