Hotspot

psaC-ndhE

SSC · 130704-131273 · 570 bp

Back to Musa

Pi 0.03
MarkerSeek score 68.40
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
570
variable_sites
117
pi
0.03
unique_haplotype_count
31
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CATAAATTTTTACTGAATGTGACA TGAAGCAGCTATTGGTCTAGC 25.0 47.6 52.3 57.8 445-973 bp 1.0 52.0
psaC-ndhE_p2 CACCCTATACATGTATCATAAATT TGAAGCAGCTATTGGTCTAGC 29.2 47.6 52.2 57.8 461-989 bp 1.0 52.0
psaC-ndhE_p3 TCATAAATTTTTACTGAATGTGAC TGAAGCAGCTATTGGTCTAGC 25.0 47.6 52.1 57.8 446-974 bp 1.0 52.0
psaC-ndhE_p4 CACCCTATACATGTATCATAAATTT TGAAGCAGCTATTGGTCTAGC 28.0 47.6 53.0 57.8 461-989 bp 1.0 52.0
psaC-ndhE_p5 CACCCTATACATGTATCATAAATTTT TGAAGCAGCTATTGGTCTAGC 26.9 47.6 53.8 57.8 461-989 bp 1.0 52.0