Hotspot

rpl32-trnL-UAG

SSC · 126480-127349 · 870 bp

Back to Musa

Pi 0.0297
MarkerSeek score 68.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
870
variable_sites
126
pi
0.0297
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.90
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTCCACCGGACGTTCAAAAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.3 57.9 649-992 bp 1.0 89.0
rpl32-trnL-UAG_p2 TTTCCACCGGACGTTCAAAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.3 57.9 650-993 bp 1.0 89.0
rpl32-trnL-UAG_p3 TTCCACCGGACGTTCAAAAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.3 57.8 642-985 bp 1.0 88.5
rpl32-trnL-UAG_p4 TTTCCACCGGACGTTCAAAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.3 57.8 643-986 bp 1.0 88.5
rpl32-trnL-UAG_p5 TTCCACCGGACGTTCAAAAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.3 57.8 647-990 bp 1.0 88.4