Hotspot

petA-psbJ

LSC · 66712-67728 · 1017 bp

Back to Musa

Pi 0.0209
MarkerSeek score 68.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1017
variable_sites
83
pi
0.0209
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGGGTCCAAGGTCTTTTGTT TCTGGGTTGGGTTCATCTCT 50.0 50.0 58.3 58.0 1125-1247 bp 1.0 87.0
petA-psbJ_p2 CGGGTCCAAGGTCTTTTGTT TGGGTTGGGTTCATCTCTCT 50.0 50.0 58.3 58.0 1123-1245 bp 1.0 87.0
petA-psbJ_p3 CGGGTCCAAGGTCTTTTGTT CTTTGGCTGGTTGGTACTGT 50.0 50.0 58.3 58.0 1197-1319 bp 1.0 86.8
petA-psbJ_p4 CGGGTCCAAGGTCTTTTGTT ATTCCTCTTTGGCTGGTTGG 50.0 50.0 58.3 58.1 1203-1325 bp 1.0 86.4
petA-psbJ_p5 CGGGTCCAAGGTCTTTTGTT GGATTCCTCTTTGGCTGGTT 50.0 50.0 58.3 57.8 1205-1327 bp 1.0 85.1