Hotspot

accD-psaI

LSC · 62707-63174 · 468 bp

Pi 0.0402

MarkerSeek score 62.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 66
pi: 0.0402
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	263-749 bp	1.0	91.6
accD-psaI_p2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	264-750 bp	1.0	91.6
accD-psaI_p3	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	427-913 bp	1.0	91.4
accD-psaI_p4	TTGCTGAACCTAACGCCTAC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	438-924 bp	1.0	90.7
accD-psaI_p5	AACGCCTACATTGCATTTGC	ATTCGGTCCCATTGGGTCTA	45.0	50.0	58.0	58.1	485-971 bp	1.0	90.2