Hotspot

rbcL-accD

LSC · 60159-60900 · 742 bp

Back to Musa

Pi 0.0194
MarkerSeek score 59.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
742
variable_sites
65
pi
0.0194
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCAAATGGAGCCCTGAACTA GGAACCACCATTTTCCCAT 50.0 47.4 57.9 55.3 833-881 bp 0.9375 56.6
rbcL-accD_p2 TAGCAAATGGAGCCCTGAAC GGAACCACCATTTTCCCAT 50.0 47.4 57.9 55.3 835-883 bp 0.9375 56.5
rbcL-accD_p3 TGAAGCTAGCAAATGGAGCC GGAACCACCATTTTCCCAT 50.0 47.4 58.2 55.3 841-889 bp 0.9375 55.6
rbcL-accD_p4 CCGTGAAGCTAGCAAATGGA GGAACCACCATTTTCCCAT 50.0 47.4 58.3 55.3 844-892 bp 0.9375 55.4
rbcL-accD_p5 TCCGTGAAGCTAGCAAATGG GGAACCACCATTTTCCCAT 50.0 47.4 58.3 55.3 845-893 bp 0.9375 55.4