Hotspot

trnG-GCC-trnfM-CAU

LSC · 39133-39278 · 146 bp

Back to Musa

Pi 0.0296
MarkerSeek score 59.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
146
variable_sites
21
pi
0.0296
unique_haplotype_count
31
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GTTCGATTCCCGCTATCCG CTTGAGGTCACGGGTTCAAA 57.9 50.0 58.2 58.0 131-300 bp 1.0 81.7
trnG-GCC-trnfM-CAU_p2 GGGTTCGATTCCCGCTATC CTTGAGGTCACGGGTTCAAA 57.9 50.0 57.8 58.0 133-302 bp 1.0 81.5
trnG-GCC-trnfM-CAU_p3 GGTTCGATTCCCGCTATCC CTTGAGGTCACGGGTTCAAA 57.9 50.0 57.8 58.0 132-301 bp 1.0 81.5
trnG-GCC-trnfM-CAU_p4 GTTCGATTCCCGCTATCCG GTCACGGGTTCAAATCCTGT 57.9 50.0 58.2 58.1 125-294 bp 1.0 81.4
trnG-GCC-trnfM-CAU_p5 GGTTCGATTCCCGCTATCC GTCACGGGTTCAAATCCTGT 57.9 50.0 57.8 58.1 126-295 bp 1.0 81.1