Hotspot

trnS-GCU-trnG-UCC

LSC · 9343-9972 · 630 bp

Back to Musa

Pi 0.0213
MarkerSeek score 68.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
630
variable_sites
104
pi
0.0213
unique_haplotype_count
37
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA AACCGAGAGACCCCTTAACT 45.0 50.0 58.0 57.7 394-1382 bp 1.0 89.1
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA ACCGAGAGACCCCTTAACTT 45.0 50.0 58.0 57.7 393-1381 bp 1.0 89.1
trnS-GCU-trnG-UCC_p3 CGGAGAGAGAGGGATTCGAA AACCGAGAGACCCCTTAACT 55.0 50.0 58.3 57.7 442-1430 bp 1.0 86.6
trnS-GCU-trnG-UCC_p4 CGGAGAGAGAGGGATTCGAA ACCGAGAGACCCCTTAACTT 55.0 50.0 58.3 57.7 441-1429 bp 1.0 86.6
trnS-GCU-trnG-UCC_p5 GCTTTAGTCCACTCAGCCAT ACCGAGAGACCCCTTAACTT 50.0 50.0 57.6 57.7 379-1367 bp 1.0 86.1