Hotspot

rps16-trnQ-UUG

LSC · 6146-7984 · 1839 bp

Back to Musa

Pi 0.0211
MarkerSeek score 63.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1839
variable_sites
188
pi
0.0211
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.89
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 904-1913 bp 1.0 90.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 915-1924 bp 1.0 89.8
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 908-1917 bp 1.0 85.5
rps16-trnQ-UUG_p4 ATCATGTCCTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.2 58.1 916-1925 bp 1.0 85.2
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 896-1905 bp 1.0 85.1