Hotspot

ycf1

SSC · 127888-133431 · 5544 bp

Back to Murraya

Pi 0.0188

MarkerSeek score 53.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5544
variable_sites: 204
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CACTTCTTCGCTTGAAAATGA	TCCTTTATTAAACGGACCTTATCG	38.1	37.5	55.1	56.6	199 bp	1.0	47.2
ycf1_p2	CACTTCTTCGCTTGAAAATGA	TGATCCTTTATTAAACGGACCT	38.1	36.4	55.1	54.9	202 bp	1.0	47.1
ycf1_p3	CACTTCTTCGCTTGAAAATGA	TGATCCTTTATTAAACGGACCTT	38.1	34.8	55.1	55.6	202 bp	1.0	47.1
ycf1_p4	CACTTCTTCGCTTGAAAATGA	ATGATCCTTTATTAAACGGACCT	38.1	34.8	55.1	55.4	203 bp	1.0	47.1
ycf1_p5	CACTTCTTCGCTTGAAAATGAT	TGATCCTTTATTAAACGGACCT	36.4	36.4	55.6	54.9	202 bp	1.0	47.1