Hotspot

ndhI-ndhA

SSC · 123675-123762 · 88 bp

Back to Murraya

Pi 0.0458

MarkerSeek score 68.10

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 7
pi: 0.0458
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	ACCGGTTACCATAGGGAACA	TTCTTCCCAACTCCTTTCGC	50.0	50.0	58.0	58.1	126-134 bp	1.0	87.2
ndhI-ndhA_p2	ACCTTGCAGCTCGTATTGTT	TTCTTCCCAACTCCTTTCGC	45.0	50.0	57.8	58.1	166-174 bp	1.0	84.8
ndhI-ndhA_p3	ACCAATGTACCTTGCAGCTC	TTCTTCCCAACTCCTTTCGC	50.0	50.0	58.2	58.1	174-182 bp	1.0	84.6
ndhI-ndhA_p4	GACCAATGTACCTTGCAGCT	TTCTTCCCAACTCCTTTCGC	50.0	50.0	58.2	58.1	175-183 bp	1.0	84.5
ndhI-ndhA_p5	TGACCAATGTACCTTGCAGC	TTCTTCCCAACTCCTTTCGC	50.0	50.0	58.5	58.1	176-184 bp	1.0	82.1