Hotspot

trnL-TAG-ccsA

SSC · 118105-118215 · 111 bp

Back to Murraya

Pi 0.0556

MarkerSeek score 75.70

Primer available yes

Label trnL-TAG-ccsA

Feature Metadata

label_name: trnL-TAG-ccsA
feature_type: igs
region: SSC
length_bp: 111
variable_sites: 10
pi: 0.0556
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-TAG-ccsA_p1	GTGAAATCGGTAGACACGCT	CTCCCGAAAGAATCCCTGTG	50.0	55.0	58.0	58.0	894-903 bp	1.0	83.8
trnL-TAG-ccsA_p2	TGAAATCGGTAGACACGCTG	CTCCCGAAAGAATCCCTGTG	50.0	55.0	58.0	58.0	893-902 bp	1.0	83.8
trnL-TAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	CTCCCGAAAGAATCCCTGTG	50.0	55.0	57.9	58.0	884-893 bp	1.0	82.9
trnL-TAG-ccsA_p4	GCGTTGAAGGCTTTTTCGTT	CTCCCGAAAGAATCCCTGTG	45.0	55.0	58.2	58.0	1455-2067 bp	1.0	82.5
trnL-TAG-ccsA_p5	GTGAAATCGGTAGACACGCT	CATTAGCCCATACTGCTCCC	50.0	55.0	58.0	57.8	909-918 bp	1.0	82.4