Hotspot

rpl32-trnL-TAG

SSC · 117554-118024 · 471 bp

Back to Murraya

Pi 0.0343

MarkerSeek score 70.20

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 471
variable_sites: 29
pi: 0.0343
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	GCGTTGAAGGCTTTTTCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	572-1190 bp	1.0	82.5
rpl32-trnL-TAG_p2	ACAGAGATAAGGACGGAGGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	57.9	58.0	1058 bp	0.25	49.3
rpl32-trnL-TAG_p3	ACAGAGATAAGGACGGAGGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	57.9	58.0	1059 bp	0.25	49.3
rpl32-trnL-TAG_p4	ACGGAGGGGCTTTTTCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1046 bp	0.25	49.3
rpl32-trnL-TAG_p5	ACGGAGGGGCTTTTTCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1047 bp	0.25	49.3