Hotspot

ndhF-rpl32

SSC · 116377-117400 · 1024 bp

Back to Murraya

Pi 0.0299

MarkerSeek score 68.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1024
variable_sites: 54
pi: 0.0299
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTTTGTTGCGGTCGGAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	1780-2383 bp	1.0	85.7
ndhF-rpl32_p2	GTTTTGTTGCGGTCGGAAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	1781-2384 bp	1.0	85.7
ndhF-rpl32_p3	GTTTTGTTGCGGTCGGAAAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	1790-2393 bp	1.0	84.7
ndhF-rpl32_p4	GCAATAGGTCGTGTGAACCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	2189-2792 bp	1.0	84.2
ndhF-rpl32_p5	GCAATAGGTCGTGTGAACCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	2190-2793 bp	1.0	84.2