Hotspot

psaJ-rpl33

LSC · 70579-71070 · 492 bp

Back to Murraya

Pi 0.0259

MarkerSeek score 69.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 25
pi: 0.0259
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGGGTCTTTAGCAGGGTTA	ACACCGTTTCGAACACAACT	50.0	45.0	58.1	58.3	630-640 bp	1.0	81.9
psaJ-rpl33_p2	TCAACCGTTTATTCCCCGAC	ACACCGTTTCGAACACAACT	50.0	45.0	57.9	58.3	600-610 bp	1.0	81.8
psaJ-rpl33_p3	TCGGGTCTTTAGCAGGGTTA	AACCCTTACTCGGACCTCTT	50.0	50.0	58.1	57.7	595-605 bp	1.0	81.8
psaJ-rpl33_p4	TCGGGTCTTTAGCAGGGTTA	ACCCTTACTCGGACCTCTTT	50.0	50.0	58.1	57.7	594-604 bp	1.0	81.8
psaJ-rpl33_p5	TCGGGTCTTTAGCAGGGTTA	ACTCGGACCTCTTTACCCTT	50.0	50.0	58.1	57.7	588-598 bp	1.0	81.8