Hotspot

atpB-rbcL

LSC · 57150-57936 · 787 bp

Back to Murraya

Pi 0.0135

MarkerSeek score 67.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 21
pi: 0.0135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	TTAACACCGGCCTTGAATCC	55.0	50.0	58.0	58.2	874-941 bp	1.0	84.1
atpB-rbcL_p2	CATCCAGTACCGGACCAATG	CCTTGAATCCAACACTCGCT	55.0	50.0	58.0	58.2	864-931 bp	1.0	84.0
atpB-rbcL_p3	TCCAGTACCGGACCAATGAT	TTAACACCGGCCTTGAATCC	50.0	50.0	58.1	58.2	872-939 bp	1.0	83.5
atpB-rbcL_p4	ATCCAGTACCGGACCAATGA	TTAACACCGGCCTTGAATCC	50.0	50.0	58.1	58.2	873-940 bp	1.0	83.4
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	CCTTGAATCCAACACTCGCT	50.0	50.0	58.1	58.2	862-929 bp	1.0	83.3