Hotspot

trnS-TGA-psbZ

LSC · 38497-38851 · 355 bp

Back to Murraya

Pi 0.0315

MarkerSeek score 57.80

Primer available yes

Label trnS-TGA-psbZ

Feature Metadata

label_name: trnS-TGA-psbZ
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 22
pi: 0.0315
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-TGA-psbZ_p1	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	418-424 bp	1.0	76.9
trnS-TGA-psbZ_p2	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGAA	55.0	38.1	58.1	57.7	418-424 bp	1.0	74.7
trnS-TGA-psbZ_p3	CAAGACCGGAGCTATCAACC	AAACAGCCAATTGGAAAGCA	55.0	40.0	58.1	56.7	413-419 bp	1.0	74.6
trnS-TGA-psbZ_p4	CAAGACCGGAGCTATCAACC	AACAGCCAATTGGAAAGCAA	55.0	40.0	58.1	56.7	412-418 bp	1.0	74.6
trnS-TGA-psbZ_p5	AAGACCGGAGCTATCAACCA	TGCAAAAACAGCCAATTGGA	50.0	40.0	58.4	57.0	417-423 bp	1.0	73.9