Hotspot

trnT-GGT-psbD

LSC · 34471-35718 · 1248 bp

Back to Murraya

Pi 0.0257
MarkerSeek score 66.00
Primer available yes
Label trnT-GGT-psbD

Feature Metadata

label_name
trnT-GGT-psbD
feature_type
igs
region
LSC
length_bp
1248
variable_sites
48
pi
0.0257
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGT-psbD_p1 GTGGTAGAGTAACGCCATGG TCCCTCCGTAACCAGTCATC 55.0 55.0 58.1 58.5 1383-1424 bp 0.75 69.1
trnT-GGT-psbD_p2 TCAGTGGTAGAGTAACGCCA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.1 58.5 1386-1427 bp 0.75 68.9
trnT-GGT-psbD_p3 GGCGTAAGTCATCGGTTCAA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.3 58.5 1360-1401 bp 0.75 67.4
trnT-GGT-psbD_p4 GTGGTAGAGTAACGCCATGG GTCCCTCCGTAACCAGTCAT 55.0 55.0 58.1 58.8 1384-1425 bp 0.75 66.8
trnT-GGT-psbD_p5 GTGGTAGAGTAACGCCATGG CCTCCGTAACCAGTCATCCA 55.0 55.0 58.1 58.8 1381-1422 bp 0.75 66.8