Hotspot

atpF-atpH

LSC · 13953-14443 · 491 bp

Back to Murraya

Pi 0.0439

MarkerSeek score 75.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 35
pi: 0.0439
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGGTCCAGCCTATTCTTGA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	880-906 bp	1.0	86.1
atpF-atpH_p2	CCAACAAATCGCGAAATGCT	ATTTCTGCCGCTTCCGTTAT	45.0	45.0	58.0	58.0	1002-1022 bp	1.0	85.6
atpF-atpH_p3	CAGCATAAAAGGGGGTTGGT	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.1	58.0	1300-1320 bp	1.0	84.6
atpF-atpH_p4	CCAACAAATCGCGAAATGCT	GTGTCTGTGGATCGAAACGA	45.0	50.0	58.0	57.9	1245 bp	0.25	49.4
atpF-atpH_p5	TGGGTCCAGCCTATTCTTGA	GTGTCTGTGGATCGAAACGA	50.0	50.0	58.0	57.9	1129 bp	0.25	49.2