Hotspot

rps16-trnQ-TTG

LSC · 6297-7571 · 1275 bp

Back to Murraya

Pi 0.0289

MarkerSeek score 68.70

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1275
variable_sites: 60
pi: 0.0289
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	CAGCGGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1170-1366 bp	1.0	87.0
rps16-trnQ-TTG_p2	GCGGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1168-1364 bp	1.0	87.0
rps16-trnQ-TTG_p3	CAGCGGATCATGTCCTTCAA	CGAAATTGAAATGGGGCGTC	50.0	50.0	58.0	58.1	1229-1425 bp	1.0	86.7
rps16-trnQ-TTG_p4	CAGCGGATCATGTCCTTCAA	GAAATTGAAATGGGGCGTCG	50.0	50.0	58.0	58.1	1228-1424 bp	1.0	86.7
rps16-trnQ-TTG_p5	GCGGATCATGTCCTTCAAGT	GAAATTGAAATGGGGCGTCG	50.0	50.0	58.0	58.1	1226-1422 bp	1.0	86.7