Hotspot

matK-rps16

LSC · 3758-5131 · 1374 bp

Back to Murraya

Pi 0.0241

MarkerSeek score 65.60

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1374
variable_sites: 60
pi: 0.0241
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CCTTCCAATGGCATCATCGA	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	1684-1744 bp	1.0	86.3
matK-rps16_p2	CCTTCCAATGGCATCATCGA	GGTGTGTTGCTGCCATTTTT	50.0	45.0	58.0	58.0	2161-2236 bp	1.0	85.9
matK-rps16_p3	AGAGGCATCCTTTACCCAGT	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	2063-2123 bp	1.0	85.5
matK-rps16_p4	AGAGGCATCCTTTACCCAGT	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	2064-2124 bp	1.0	85.5
matK-rps16_p5	AGAGGCATCCTTTACCCAGT	GGTGTGTTGCTGCCATTTTT	50.0	45.0	58.0	58.0	2540-2615 bp	1.0	85.3