Hotspot

trnH-GTG-psbA

LSC · 89-536 · 448 bp

Back to Murraya

Pi 0.036

MarkerSeek score 70.70

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 29
pi: 0.036
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	GATCCACTTGGCTACATCCG	TGAAGCCCCATCTACAAATGG	55.0	47.6	58.1	58.0	494-523 bp	1.0	77.4
trnH-GTG-psbA_p2	CAATCCACTGCCTTGATCCA	TGAAGCCCCATCTACAAATGG	50.0	47.6	57.9	58.0	508-537 bp	1.0	77.2
trnH-GTG-psbA_p3	AATCCACTGCCTTGATCCAC	TGAAGCCCCATCTACAAATGG	50.0	47.6	57.9	58.0	507-536 bp	1.0	77.2
trnH-GTG-psbA_p4	ACAATCCACTGCCTTGATCC	TGAAGCCCCATCTACAAATGG	50.0	47.6	57.9	58.0	509-538 bp	1.0	77.1
trnH-GTG-psbA_p5	CGCATGGTGGATTCACAATC	TGAAGCCCCATCTACAAATGG	50.0	47.6	57.5	58.0	523-552 bp	1.0	74.2