Hotspot

psbA

LSC · 323-1384 · 1062 bp

Back to Murbeckiella

Pi 0

MarkerSeek score 29.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGAAGGCGTATATTGCTCGT	AGAATTTGTGCGCTTGGGA	45.0	47.4	56.8	58.0	1171 bp	1.0	62.3
psbA_p2	AGAAGGCGTATATTGCTCGT	AGAATTTGTGCGCTTGGGAG	45.0	50.0	56.8	59.1	1171 bp	1.0	61.6
psbA_p3	AGAAGGCGTATATTGCTCGT	AGAGAATTTGTGCGCTTGGG	45.0	50.0	56.8	59.1	1173 bp	1.0	61.6
psbA_p4	AGAAGGCGTATATTGCTCGT	GAGAATTTGTGCGCTTGGGA	45.0	50.0	56.8	59.1	1172 bp	1.0	61.6
psbA_p5	AGAAGGCGTATATTGCTCGT	GCTTGGGAGTCCCTGATGAT	45.0	55.0	56.8	59.2	1160 bp	1.0	61.3