Hotspot

ndhF-rpl32

SSC · 112784-113620 · 837 bp

Back to Murbeckiella

Pi 0.00598

MarkerSeek score 62.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 837
variable_sites: 5
pi: 0.00598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGTACGGGAAGTGGAATGA	ACGATGTCCAATATCCCTTCC	50.0	47.6	58.1	57.2	979-984 bp	1.0	65.9
ndhF-rpl32_p2	ACGGGAAGTGGAATGAAAGG	ACGATGTCCAATATCCCTTCC	50.0	47.6	57.8	57.2	975-980 bp	1.0	64.7
ndhF-rpl32_p3	AGGTACGGGAAGTGGAATGA	CGATGTCCAATATCCCTTCCT	50.0	47.6	58.1	56.9	978-983 bp	1.0	63.6
ndhF-rpl32_p4	AGGTACGGGAAGTGGAATGA	AACGATGTCCAATATCCCTTCC	50.0	45.5	58.1	57.9	980-985 bp	1.0	63.4
ndhF-rpl32_p5	AGGTACGGGAAGTGGAATGA	AAGCTTTCAACGATGTCCAA	50.0	40.0	58.1	55.9	988-993 bp	1.0	63.1