Hotspot

trnR-CGU-trnN-AAC

IRb · 108534-109163 · 630 bp

Back to Murbeckiella

Pi 0.00476

MarkerSeek score 61.50

Primer available yes

Label trnR-CGU-trnN-AAC

Feature Metadata

label_name: trnR-CGU-trnN-AAC
feature_type: igs
region: IRb
length_bp: 630
variable_sites: 3
pi: 0.00476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-CGU-trnN-AAC_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	771 bp	1.0	78.6
trnR-CGU-trnN-AAC_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	808 bp	1.0	78.0
trnR-CGU-trnN-AAC_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	812 bp	1.0	77.8
trnR-CGU-trnN-AAC_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	722 bp	1.0	77.4
trnR-CGU-trnN-AAC_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	849 bp	1.0	77.2