Hotspot

rpl20-rps12

LSC · 68720-69472 · 753 bp

Back to Murbeckiella

Pi 0.00398

MarkerSeek score 61.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 3
pi: 0.00398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGTGAATGAGCTCCTCGAAA	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	926 bp	1.0	78.1
rpl20-rps12_p2	TGAATGAGCTCCTCGAAAGC	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	924 bp	1.0	78.0
rpl20-rps12_p3	CGTGAATGAGCTCCTCGAAA	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	865 bp	1.0	77.9
rpl20-rps12_p4	TGAATGAGCTCCTCGAAAGC	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	863 bp	1.0	77.8
rpl20-rps12_p5	TTTTGTTCTACGCCTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	885 bp	1.0	77.5