Hotspot

psaJ-rpl33

LSC · 66904-67332 · 429 bp

Back to Murbeckiella

Pi 0.021

MarkerSeek score 63.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 9
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCGGTTCGTTAGCAGGTTT	TCTGGAAATTCCCGCTGATT	45.0	45.0	57.8	57.2	606 bp	1.0	72.8
psaJ-rpl33_p2	TTCGGTTCGTTAGCAGGTTT	TGGAAATTCCCGCTGATTCT	45.0	45.0	57.8	57.2	604 bp	1.0	72.8
psaJ-rpl33_p3	ATGGTTCGGTTCGTTAGCAG	TCTGGAAATTCCCGCTGATT	50.0	45.0	58.3	57.2	610 bp	1.0	72.5
psaJ-rpl33_p4	ATGGTTCGGTTCGTTAGCAG	TGGAAATTCCCGCTGATTCT	50.0	45.0	58.3	57.2	608 bp	1.0	72.5
psaJ-rpl33_p5	TTCGGTTCGTTAGCAGGTTT	AACATCTTTACCCTTGGCCA	45.0	45.0	57.8	57.0	531 bp	1.0	71.5