Hotspot

trnC-UGC-petN

LSC · 27268-27929 · 662 bp

Back to Murbeckiella

Pi 0.0031

MarkerSeek score 62.90

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 2
pi: 0.0031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	761-772 bp	1.0	81.2
trnC-UGC-petN_p2	GTTTCTATCGTTTTGGCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	817-828 bp	1.0	80.8
trnC-UGC-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	762-773 bp	1.0	80.6
trnC-UGC-petN_p4	GTTTCTATCGTTTTGGCGGC	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	818-829 bp	1.0	80.2
trnC-UGC-petN_p5	TTTCCCCAGTTCAAATCCGG	TTCCCCACACTACGAGAGAG	50.0	55.0	58.1	58.2	756-767 bp	1.0	79.9