Hotspot

rpoB-trnC-UGC

LSC · 26108-27196 · 1089 bp

Back to Murbeckiella

Pi 0.00461

MarkerSeek score 61.80

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1089
variable_sites: 5
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	CCGGGTATTGTAGATGTTCCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	57.9	58.1	1189-1192 bp	1.0	71.0
rpoB-trnC-UGC_p2	GTTCCCTCTTTTTCATCCCCA	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	1174-1177 bp	1.0	70.6
rpoB-trnC-UGC_p3	TGTTCCCTCTTTTTCATCCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.8	58.1	1175-1178 bp	1.0	70.6
rpoB-trnC-UGC_p4	CCGGGTATTGTAGATGTTCCC	AAGTCTGTTATTGAGGCGGC	52.4	50.0	57.9	58.3	1211-1214 bp	1.0	69.5
rpoB-trnC-UGC_p5	CCTCTTTTTCATCCCCAAGC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.7	58.1	1170-1173 bp	1.0	69.3