Hotspot

ndhF

SSC · 113127-115373 · 2247 bp

Back to Mukdenia

Pi 0.00312

MarkerSeek score 45.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 7
pi: 0.00312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAACCTCTTGTGACCCGTC	ACGGATATCATAGCGTCTCCT	50.0	47.6	58.0	57.9	2869-2900 bp	1.0	71.4
ndhF_p2	CTTGTGACCCGTCTTTTCGA	ACGGATATCATAGCGTCTCCT	50.0	47.6	58.1	57.9	2862-2893 bp	1.0	70.6
ndhF_p3	TCTTGTGACCCGTCTTTTCG	ACGGATATCATAGCGTCTCCT	50.0	47.6	58.1	57.9	2863-2894 bp	1.0	70.6
ndhF_p4	CCTCTTGTGACCCGTCTTTT	ACGGATATCATAGCGTCTCCT	50.0	47.6	57.7	57.9	2865-2896 bp	1.0	69.7
ndhF_p5	AAAACCTCTTGTGACCCGTC	GACGGATATCATAGCGTCTCC	50.0	52.4	58.0	57.6	2870-2901 bp	1.0	68.8