Hotspot

ndhD

SSC · 118546-120048 · 1503 bp

Back to Mukdenia

Pi 0.00333

MarkerSeek score 47.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 5
pi: 0.00333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAAGCCGACGAGAACCATT	TGCTTCTGCTCCAAGAACAG	50.0	50.0	58.0	58.1	1957-1958 bp	1.0	79.6
ndhD_p2	GTAAGCCGACGAGAACCATT	TGATACCTTGGGACGGATGT	50.0	50.0	58.0	58.1	1991-1992 bp	1.0	79.5
ndhD_p3	GTAAGCCGACGAGAACCATT	CTCATGAGCCGATCCAGAAG	50.0	55.0	58.0	57.8	2209-2210 bp	1.0	79.2
ndhD_p4	GTAAGCCGACGAGAACCATT	GCTTCTGCTCCAAGAACAGA	50.0	50.0	58.0	57.8	1956-1957 bp	1.0	79.1
ndhD_p5	CAAATTCCGCAATTGTGGCT	TGCTTCTGCTCCAAGAACAG	45.0	50.0	57.9	58.1	2131-2132 bp	1.0	79.0