Hotspot

ccsA

SSC · 117329-118285 · 957 bp

Back to Mukdenia

Pi 0.00418

MarkerSeek score 50.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 957
variable_sites: 4
pi: 0.00418
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CGGCATGGGATCCTCTAAAA	TCGTAAAGCCCTTTCTGCAA	50.0	45.0	57.4	57.7	1093 bp	1.0	73.1
ccsA_p2	CGGCATGGGATCCTCTAAAA	TTCGTAAAGCCCTTTCTGCA	50.0	45.0	57.4	57.7	1094 bp	1.0	73.1
ccsA_p3	ATTCCCGATTTCCATTCCGT	TCGTAAAGCCCTTTCTGCAA	45.0	45.0	57.3	57.7	1044 bp	1.0	72.4
ccsA_p4	ATTCCCGATTTCCATTCCGT	TTCGTAAAGCCCTTTCTGCA	45.0	45.0	57.3	57.7	1045 bp	1.0	72.4
ccsA_p5	AATTCCCGATTTCCATTCCGT	TCGTAAAGCCCTTTCTGCAA	42.9	45.0	58.0	57.7	1045 bp	1.0	70.1