Hotspot

ndhI-ndhA

SSC · 122674-122749 · 76 bp

Back to Mukdenia

Pi 0.0139

MarkerSeek score 65.20

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 76
variable_sites: 1
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	AACCCGGTTACCATAGGGAA	CTTCCCAACTCCTTTCACTGT	50.0	47.6	58.0	57.8	118-122 bp	1.0	73.4
ndhI-ndhA_p2	AACCCGGTTACCATAGGGAA	ACCTAGGCTAAGAATGGACCA	50.0	47.6	58.0	57.9	204-208 bp	1.0	72.7
ndhI-ndhA_p3	TGCAGCTCGTACTGTTTGTT	CTTCCCAACTCCTTTCACTGT	45.0	47.6	58.1	57.8	152-156 bp	1.0	72.5
ndhI-ndhA_p4	TGCAGCTCGTACTGTTTGTT	ACCTAGGCTAAGAATGGACCA	45.0	47.6	58.1	57.9	238-242 bp	1.0	72.2
ndhI-ndhA_p5	TTGCAGCTCGTACTGTTTGT	ACCTAGGCTAAGAATGGACCA	45.0	47.6	58.1	57.9	239-243 bp	1.0	72.2