Hotspot

ycf1-ndhF

SSC · 113105-113126 · 22 bp

Back to Mukdenia

Pi 0.0455

MarkerSeek score 65.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 22
variable_sites: 1
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AAAACCTCTTGTGACCCGTC	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.0	58.0	693-724 bp	1.0	80.1
ycf1-ndhF_p2	GCAGAATACCGTCACCCATT	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.0	58.0	1084-1115 bp	1.0	79.9
ycf1-ndhF_p3	AAGAGGGATCCACCGAAGAA	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.0	58.0	952-983 bp	1.0	79.9
ycf1-ndhF_p4	GGGAAAGCGAGGAAGAAACA	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.1	58.0	1021-1052 bp	1.0	79.4
ycf1-ndhF_p5	AAAACCTCTTGTGACCCGTC	TTGTTCGTTGGAGCCATAGG	50.0	50.0	58.0	57.9	690-721 bp	1.0	79.4