Hotspot

petA-psbJ

LSC · 66312-67042 · 731 bp

Back to Mukdenia

Pi 0.00687

MarkerSeek score 48.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 5
pi: 0.00687
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1362-1365 bp	1.0	80.1
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	ACGACACAATCAAACCCGAA	50.0	45.0	58.0	58.0	1365-1368 bp	1.0	80.1
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1595-1598 bp	1.0	80.0
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1455-1458 bp	1.0	80.0
petA-psbJ_p5	CAGGGCCAGAACTTCTTGTT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1453-1456 bp	1.0	80.0