Hotspot

petN-psbM

LSC · 29987-30654 · 668 bp

Back to Mukdenia

Pi 0.0195

MarkerSeek score 68.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 13
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	830-842 bp	1.0	68.8
petN-psbM_p2	GGGGACACAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	852-864 bp	1.0	68.2
petN-psbM_p3	AGGGGACACAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	853-865 bp	1.0	68.2
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	769-781 bp	1.0	67.3
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	830-842 bp	1.0	64.6