Hotspot

trnK-UUU-rps16

LSC · 4325-4946 · 622 bp

Back to Mukdenia

Pi 0.00483

MarkerSeek score 61.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 3
pi: 0.00483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCCTTGCTTCATCCA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	799-805 bp	1.0	79.9
trnK-UUU-rps16_p2	ACGAATCCCTTGCTTCATCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	800-806 bp	1.0	79.9
trnK-UUU-rps16_p3	ATGGACGAATCCCTTGCTTC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	804-810 bp	1.0	79.8
trnK-UUU-rps16_p4	GGACGAATCCCTTGCTTCAT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	802-808 bp	1.0	79.8
trnK-UUU-rps16_p5	GTCGCGGTCTTACAAACTCT	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.9	58.0	833-839 bp	1.0	79.7