Hotspot

ccsA-ndhD

SSC · 115922-116224 · 303 bp

Back to Muiriantha

Pi 0.0698

MarkerSeek score 72.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 303
variable_sites: 21
pi: 0.0698
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGATTACATAGTTATGGTTCA	AGTGGACAAGACCGAAGCTA	31.8	50.0	52.0	58.4	374 bp	1.0	44.9
ccsA-ndhD_p2	GGAATAGGATTACATAGTTATGGTTCA	AGTGGACAAGACCGAAGCTA	33.3	50.0	56.8	58.4	379 bp	1.0	44.9
ccsA-ndhD_p3	AGGATTACATAGTTATGGTTCA	GTGGACAAGACCGAAGCTAT	31.8	50.0	52.0	57.3	373 bp	1.0	44.9
ccsA-ndhD_p4	AGGATTACATAGTTATGGTTCA	TCCGGATTTCGTTCTCTCAC	31.8	50.0	52.0	57.4	398 bp	1.0	44.6
ccsA-ndhD_p5	AGGATTACATAGTTATGGTTCA	CCGGATTTCGTTCTCTCACT	31.8	50.0	52.0	57.3	397 bp	1.0	44.6