Hotspot

psbE-petL

LSC · 65190-66577 · 1388 bp

Back to Muiriantha

Pi 0.0225

MarkerSeek score 67.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1388
variable_sites: 31
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAAGCGGCTAGGAGAAAACC	50.0	50.0	57.9	58.2	1530-1536 bp	1.0	80.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAAGCGGCTAGGAGAAAACC	50.0	50.0	57.8	58.2	1453-1459 bp	1.0	79.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAAGCGGCTAGGAGAAAACC	45.0	50.0	58.2	58.2	1539-1545 bp	1.0	79.3
psbE-petL_p4	TGCTGACGAATAACCAACCC	AAAGCGGCTAGGAGAAAACC	50.0	50.0	58.2	58.2	1544-1550 bp	1.0	79.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	AAAGCGGCTAGGAGAAAACC	50.0	50.0	57.8	58.2	1538-1544 bp	1.0	79.2