Hotspot

accD-psaI

LSC · 59144-59853 · 710 bp

Back to Muiriantha

Pi 0.0305

MarkerSeek score 74.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 21
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TATTGCAGTTCCACGCCTTT	AAAGAAGCCATTGCCATTGC	45.0	45.0	58.1	57.9	840-861 bp	1.0	82.4
accD-psaI_p2	TTATTGCAGTTCCACGCCTT	AAAGAAGCCATTGCCATTGC	45.0	45.0	58.1	57.9	841-862 bp	1.0	82.4
accD-psaI_p3	TATTGCAGTTCCACGCCTTT	TTGCCATTGCCGGAAATACT	45.0	45.0	58.1	58.2	830-851 bp	1.0	82.0
accD-psaI_p4	TTATTGCAGTTCCACGCCTT	TTGCCATTGCCGGAAATACT	45.0	45.0	58.1	58.2	831-852 bp	1.0	82.0
accD-psaI_p5	TATTGCAGTTCCACGCCTTT	ATGGAGGGAAAGCTGTTGAG	45.0	50.0	58.1	57.5	789-810 bp	1.0	79.4