Hotspot

petN-psbM

LSC · 28867-30185 · 1319 bp

Back to Muiriantha

Pi 0.0262
MarkerSeek score 69.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1319
variable_sites
34
pi
0.0262
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACATTTTCCCTTTCGCTCGT TCGTTCTAGTTCCTACTGCCT 45.0 47.6 58.1 57.9 1413-1429 bp 1.0 73.6
petN-psbM_p2 CCCTTTCGCTCGTAGTATGG TCGTTCTAGTTCCTACTGCCT 55.0 47.6 57.9 57.9 1406-1422 bp 1.0 73.4
petN-psbM_p3 CCTTTCGCTCGTAGTATGGG TCGTTCTAGTTCCTACTGCCT 55.0 47.6 57.9 57.9 1405-1421 bp 1.0 73.4
petN-psbM_p4 CTTTCGCTCGTAGTATGGGG TCGTTCTAGTTCCTACTGCCT 55.0 47.6 57.9 57.9 1404-1420 bp 1.0 73.4
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT TCGTTCTAGTTCCTACTGCCT 50.0 47.6 58.2 57.9 1451-1467 bp 1.0 73.1