Hotspot

atpF-atpH

LSC · 12601-13117 · 517 bp

Back to Muiriantha

Pi 0.038

MarkerSeek score 79.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 517
variable_sites: 19
pi: 0.038
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCAGTGAACCAAAGAAACG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	663-673 bp	1.0	77.9
atpF-atpH_p2	GCCAGTGAACCAAAGAAACG	CGAGGCGGAGGGAAAAATAC	50.0	55.0	57.9	58.7	668-678 bp	1.0	77.7
atpF-atpH_p3	GCCAGTGAACCAAAGAAACG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	667-677 bp	1.0	77.7
atpF-atpH_p4	GCCAGTGAACCAAAGAAACG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	664-674 bp	1.0	77.7
atpF-atpH_p5	GCCAGTGAACCAAAGAAACG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	665-675 bp	1.0	77.7