Hotspot

atpA-atpF

LSC · 11200-11258 · 59 bp

Back to Muiriantha

Pi 0.0678

MarkerSeek score 72.70

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 4
pi: 0.0678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TGACATATTTGGGGCGATGA	55.0	45.0	58.1	57.0	209 bp	1.0	72.6
atpA-atpF_p2	GTCGCCTACTTGAAGTACGG	TGACATATTTGGGGCGATGA	55.0	45.0	58.1	57.0	207 bp	1.0	72.6
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	TGACATATTTGGGGCGATGAA	55.0	42.9	58.1	57.7	209 bp	1.0	70.5
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	TGACATATTTGGGGCGATGAA	55.0	42.9	58.1	57.7	207 bp	1.0	70.5
atpA-atpF_p5	GTCGCCTACTTGAAGTACGG	TTGACATATTTGGGGCGATGA	55.0	42.9	58.1	57.7	208 bp	1.0	70.5